New Product Release
ImmunoHistoProbe® Plus
Double Staining Polymer IHC Detection System (High Sensitivity)
Introducing the New Generation ImmunoHistoProbe® Plus Double Staining Polymer IHC Detection Systems! Unleash the power of simultaneous detection with our cutting-edge technology, offering higher sensitivity and an easy protocol.
Protocol is as simple as single staining
New Generation ImmunoHistoProbe® Plus Double Staining Polymer IHC Detection System (High Sensitivity) Cat No. 8981-09 and 8982-09 has superior sensitivity than other regular polymer detection system.
Stronger staining and higher sensitivity was observed when use ImmunoHistoProbe® Plus High Sensitivity Double Staining Polymer IHC Detection System (Pictures B, D, F) comparing with regular one step polymer detection system (Pictures A, C, E).
Archival formalin-fixed paraffin embedded human tissue sections were deparaffinized in xylene with 2 changes for 5 minutes each. Rehydrate through graded alcohol (100%, 95% and 70%). 1mM EDTA pH9.0 HIER 15’ or none. New ImmunoHistoProbe® Plus High Sensitivity Double Staining Polymer IHC Detection System was incubated @RT 15min+15min: Amplifier 15min, wash 3 x 2 min then add HRP/AP Polymer Detector for another 15 min. (Picture B, D, F). HRP Polymer Goat anti-Rabbit or mouse and AP Polymer Goat anti-Mouse or rabbit (1:1 mixture) was incubated @RT 20 min (Picture A, C, E). DAB Chromogen and AP-Red Chromogen. Hematoxylin counter staining. Serial tissue sections were used in order to compare staining intensity side by side.
A and B: Esophageal Carcinoma. Mouse anti-P53 (1:20,000) and Rabbit anti-CD146 (1:1000) cocktail, RT, 2 hr.
C and D: Tonsil, Mouse anti-Ki67 (1:10,000) and Rabbit anti-CD3 cocktail, RT, 2 hrs.
E and F: Colon Cancer, No HIER. Mouse anti-Smooth Muscle Actin (1:5000) and Rabbit anti-CEA (1:2000) cocktail, RT, 2 hrs.
Experience the future of double staining with the Next Generation ImmunoHistoProbe® Plus High Sensitivity Double Staining Polymer IHC Detection Systems. Upgrade your research today and unlock the full potential of simultaneous double staining as easy as single staining. Contact us now to learn more about our innovative detection system. Experience the power of our new-generation IHC detection with an easy protocol, stronger staining, and higher sensitivity!
Ordering information:
Cat No. | Product Description | Size |
8981-09 | ImmunoHistoProbe® Plus Double Staining Polymer IHC Detection System (High Sensitivity) (Polymer-AP for Mouse and Polymer-HRP for Rabbit) | 1000ml |
8982-09 | ImmunoHistoProbe® Plus Double Staining Polymer IHC Detection System (High Sensitivity) (Polymer-HRP for Mouse and Polymer-AP for Rabbit) | 1000ml |