Mice have been widely used in biotechnology and medical research for over a century, particularly in the production of monoclonal primary antibodies. However, researchers studying cancer and pathology often struggle to stain mouse primary antibodies on mouse tissue due to limited options of primary antibodies other than mouse-originated. The main issue is that anti-mouse secondary antibodies cannot distinguish between endogenous mouse immunoglobulins and mouse primary antibodies when using the indirect method, leading to high background staining that obscures specific staining. This problem varies depending on the tissue type and the sample, making it challenging for researchers. Although there are commercially available IHC detection kits, their results are not always satisfactory.

To address this challenge, Advanced Biosystems developed the ImmunoHistoProbe® Mouse on Mouse Polymer IHC Detection Kit (Cat No. 3851-07). The kit uses patented technology (pending approval) to eliminate background interference. The detection reagent only binds to primary antibodies and not to endogenous immunoglobulins, thus completely avoiding background caused by endogenous IgG without the need for blocking reagents. Additionally, the system is biotin-free, eliminating endogenous biotin background in biotin-rich tissues. By using the ImmunoHistoProbe® Mouse on Mouse Polymer Detection Kit, researchers can expect superior signal-to-noise ratio compared to other kits on the market.